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KMID : 0616120100410030221
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Korean Journal of Pharmacognosy
2010 Volume.41 No. 3 p.221 ~ p.226
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Enhancement of Dopamine Biosynthesis by Sesamin in PC12 Cells
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Zhang Min
Choi Hyun-Sook
Lee Myung-Koo
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Abstract
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The effects of sesamin on dopamine biosynthesis in PC12 cells were investigated. Sesamin at concentration ranges of 20-75 significantly increased intracellular dopamine levels and tyrosine hydroxylase (TH) activities at 24 h: 50 sesamin increased dopamine levels to 132% and TH activities to 128% of control levels. Sesamin (50 ) induced the phosphorylation of TH, cyclic AMP-dependent protein kinase (PKA) and cyclic AMP-response element binding protein (CREB) for 0.5-24 h. Sesamin (50 ) also increased the mRNA levels of TH and CREB for 3-24 h. In addition, sesamin (50 ) associated with L-DOPA (50 and 100 ) further increased the intracellular levels of dopamine for 24 h compared to L-DOPA alone. These results suggest that sesamin enhances dopamine biosynthesis and L-DOPA-induced increase in dopamine levels by inducing TH activity and TH gene expression, which is mediated by PKA-CREB systems in PC12 cells. Therefore, sesamin could serve as an adjuvant phytonutrient for neurodegenerative diseases.
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KEYWORD
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Sesamin, dopamine levels, tyrosine hydroxylase, PKA, CREB, PC12 cells
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